supplemented medium

The conditioned medium should be prepared by culturing 2~ 4 days of thecal cells in the medium supplemented with 10% serum but without hormones.

条件液制备前应加入10%的血清，不加促性腺激素。

CONCLUSION: The keratinocytes growed best in serum-free medium supplemented with EGF and BPE.

结论: 细胞在加入表皮生长因子和促进增殖的垂体提取物的无血清培养基中增殖良好.

The callus induction medium supplemented with two auxins ( 2,4-D and NAA) was much better than that with 2,4-D individual.

在愈伤诱导培养基中同时使用两种生长激素（2,4D和NAA）的效果要好于单独使用一种生长激素（2,4D）的效果；

Somatic embryogenesis were obtained from mature embryo axes of eleven Arachis hypogaea varieties cultured on MS medium supplemented with 2,4D.

用11个不同花生品种的胚轴为外植体，接种于含2,4D的MS培养基进行体细胞胚的诱导。